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Home Article How can the Royal qPCR Microbial Detector overcome the time bottleneck of traditional methods?

How can the Royal qPCR Microbial Detector overcome the time bottleneck of traditional methods?

Technical articles 2025-11-18

In the fields of food safety control and clinical diagnosis,the timeliness of microbiological testing directly determines the efficiency of risk prevention and control. The traditional microbial cultivation method requires a complete process of"enrichment separation identification",and the detection often takes 24-72 hours to complete,which is difficult to meet the rapid response needs of sudden pollution incidents. TheRoyal qPCR Microbial Detectorinnovates its technological path by starting with the reconstruction of detection principles,shortening the detection time to several hours or even tens of minutes,completely breaking through the time constraints of traditional methods.

The core logic of breaking through the time bottleneck is to skip the long process of"microbial proliferation to visible colonies"in traditional cultivation methods and directly capture the characteristic signals of microorganisms. Although the principles of detectors with different technological routes vary,they all revolve around"accurately identifying the inherent properties of microorganisms". Among them,immunochromatography technology captures microbial antigens through specific antibodies,combines colloidal gold labeling to achieve visual detection,and utilizes the rapid binding characteristics of antigen antibody reactions to compress the detection time to 15-30 minutes. It is widely used for rapid screening of common pathogenic bacteria such as Salmonella and Escherichia coli.

The application of molecular biology technology has achieved more accurate and rapid detection. A PCR(polymerase chain reaction)based detector can perform targeted amplification of unique microbial gene fragments. The amplification products can be monitored in real-time using fluorescent probes,and qualitative and quantitative analysis can be completed within 2-4 hours. And digital PCR technology further enhances sensitivity,even if the sample contains only a few microorganisms,accurate counting can be achieved through droplet partition amplification,solving the dual problems of"low concentration sample missed detection"and"long time consumption"in traditional methods.

Biosensor technology constructs a detection path of"direct signal conversion". Its core is to combine biometric elements(such as enzymes and bacteriophages)with physical sensors. When microorganisms interact with the recognition elements,it will cause changes in electrical and optical signals,and the sensors will convert these changes into quantifiable data. This type of detector has an extremely fast response speed,with some models only taking 1 hour to detect Staphylococcus aureus,and does not require complex sample preprocessing,making it particularly suitable for on-site rapid detection scenarios.

Technological innovation has also driven the expansion of application scenarios. In the food processing industry,the rapid detection instrument next to the production line can achieve"instant sampling and instant detection",avoiding unqualified products from entering the market; In the clinical field,rapid testing results can help doctors develop anti infective treatment plans earlier and reduce the risk of severe illness. It should be noted that different technologies have their own focuses:immunochromatography is suitable for rapid screening,PCR is suitable for precise identification,and in practical applications,it needs to be selected according to the detection requirements. The development of the Royal qPCR microbiological detector is reshaping the industry standard for microbiological testing with its technological advantages of"fast,accurate,and stable",providing strong support for public safety and health protection.

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