Benchtop Cell Smear Centrifuge-Huatai Hehe (Beijing) Trading Co., Ltd

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Benchtop Cell Smear Centrifuge

Large-screen true-color LCD display with full touchscreen operation interface; microcomputer control, driven by a brushless AC variable-frequency motor, featuring high speed accuracy and low noise; programmable operation with multiple speed-up and speed-down options; independent setting and on-screen simultaneous display of centrifugal force and speed; unique DCT [Differential Centrifugation Technology] ‘Differential Centrifugation Application Technology‘. Widely applicable to: education, scientific research, biochemical products, medical testing, food safety, agricultural product testing, animal husbandry and aquatic product testing, microbiology, molecular chemistry, environmental protection, quality inspection and other industries.

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01Product Description

Widely applicable to: education, scientific research, biochemical products, medical testing, food safety, agricultural product testing, animal husbandry and aquatic product testing, microbiology, molecular chemistry, environmental protection, quality inspection and other industries.

1. Sample Pretreatment and Loading Logic

The cell sample to be processed (typically a liquid suspension with a concentration adapted to the instrument requirements, generally 10 ⁶~10⁸ cells/mL) must first be mixed with an anticoagulant (e.g., EDTA, heparin) to prevent cell aggregation. Subsequently, the sample is injected into a specialized centrifuge tube (or sample well), and the glass slide is fixed onto the centrifuge’s specialized rotor body (such as the "specialized rotor body for cell smears" mentioned earlier). It is crucial to ensure the glass slide is parallel to the sample liquid surface with a uniform gap (usually 0.1~0.3mm) — this design serves as the foundation for the subsequent uniform spreading of cells, avoiding uneven smear thickness caused by gap variations.

2. Directional Driving Effect of Centrifugal Force

After the instrument is started, the rotor rotates at a set speed (common range: 200~2000 rpm, corresponding to a relative centrifugal force (RCF) of 50~720 xg, as per the previous parameter "2000rpm 720xg"), generating a radially outward centrifugal force. At this point, due to their significantly higher density than the liquid medium (e.g., plasma, culture medium), the cells in the sample overcome the viscous resistance of the liquid under the action of centrifugal force and undergo directional sedimentation toward the glass slide. The magnitude of the centrifugal force directly determines the cell sedimentation rate: an excessively low RCF results in incomplete cell sedimentation and sparse smears, while an excessively high RCF may cause cell lysis and morphological deformation. Therefore, parameters must be precisely matched according to the cell type (e.g., 1000~1500 rpm for blood cell smears and 800~1200 rpm for tumor cell smears).

3. Core Mechanism of Uniform Cell Spreading

When cells sediment onto the glass slide surface, the rotational movement of the rotor also generates tangential frictional force. Combined with the flow-guiding design of the sample well (wedge-shaped wells in some instruments), this force causes cells to form a gradient distribution (or uniform thin layer) from dense to sparse along the centrifugal direction on the glass slide. This process is analogous to "centrifugal force driving liquid flow to 'brush' cells onto the glass slide," ultimately forming a cell smear with a thickness of 1~5μm. The thickness can be adjusted by modifying the rotation speed and centrifugation time: higher speeds and longer durations result in denser smears, though cell stacking must be avoided.

4. Connection Between Termination and Sample Fixation

Upon reaching the set centrifugation time (typically 30~120 seconds; short-term centrifugation minimizes cell morphological damage), the instrument smoothly stops the rotor via its braking system (to prevent smear detachment or cell displacement due to inertia). At this stage, the cells have been fixed to the glass slide surface through physical adsorption and can be directly removed for subsequent processing (e.g., methanol fixation, HE staining, immunohistochemical staining). Finally, the smear is used for pathological diagnosis, cell morphological analysis, or scientific research observations.

Adaptability Between Key Designs and Principles

▲ Specialized Rotor Body: Station designs such as "2 plates × 6" and "12 plates" (as referenced in previous parameters) enable simultaneous processing of multiple sample groups. They ensure centrifugal force consistency across all stations (error ≤ ±5%), guaranteeing the uniformity of batch-prepared smears.
▲Precise Control of Rotation Speed and RCF: The instrument adjusts the rotation speed via a variable-frequency motor to stabilize the RCF within the target range, avoiding variations in cell smear quality caused by centrifugal force fluctuations.
▲Contamination Prevention Design: The parts of the rotor in contact with samples are made of inert materials (e.g., stainless steel, PTFE coating), and the sample wells are independently separated to prevent cross-contamination, meeting the sterility requirements of clinical testing.

Model	JW-2018 Cell Smear	JW-2028 Cell Smear	JW-2038 Milk Fat	JW-2040 PRP
Max Speed(rpm)	2000rpm	1500rpm	6000rpm	4500rpm
Max. RCF	720xg	423xg	7164xg	3894xg
Capacity	12Film:2piecex6	18piece	50mlx4	100mlx8
Speed accuracy	±10rpm
Timing range	1s~99h59min59s / Pulse (instantaneous) centrifugation
Test solution temperature rise	The temperature rise of the test solution shall be less than 10℃after 20 minutes of operation.
Noise	≤60dB
power supply	AC220V 50Hz10A
Power	400W
Weight	47Kg	55Kg	60Kg	60Kg
Overall dimensions [L x W x H]	540x425x400mm	580x465x400mm	580x465x400mm	580x465x400mm

Model Rotor	JW-2018Cell Smear	JW-2028Cell Smear	JW-2038Milk Fat	JW-2040 PRP
NO1: Specialized Rotor Body for Cell Smear	12-Plate (2000 rpm, 720 xg)	8-plates（1500rpm ,423xg）	/	/
	2 plates×6	18plates
NO2: Gerber Tube Rotor	/	/	30mlx6 6000rpm 7164xg
NO3: Pasteurized Milk Fat Bottle Rotor	/	/	50mlx6 4000rpm 3077xg
NO4: Horizontal rotor	/	/	/	100mlx2 4500rpm 3645xg
				Specialized PRP Tubes for Aesthetics and Orthopedics
NO5: Horizontal rotor	/	/	/	100mlx4 4500rpm 3894xg
				Specialized PRP Tubes for Aesthetics and Orthopedics
				Adapter1: 50mlx4
				Compatible with:50ml Syringes
				Adapter2: 20mlx4
				Compatible with:200ml Syringes
				Adapter3:10mlx16
				Compatible with:10ml Syringes
NO6： Horizontal rotor	/	/	/	100mlx8 4500rpm 3894xg
				Specialized PRP Tubes for Aesthetics and Orthopedics
				Adapter：50mlx8 Syringes
				Adapter：20mlx8
				Compatible with 20 mL Syringes

02Product Advantage

◆ Microcomputer control, large screen true color LCD display, full touch screen operation interface; Directly set the working program, centrifugal force, speed, centrifugal time, lifting speed, and the rotor model LCD display on the same screen

◆ Carbon brushless AC variable frequency motor drive, using a special drive module to ensure the reliability of the circuit

◆ Double door lock system, automatically lock the door after starting, to ensure the safety of operation

◆20 kinds of work program selection, users can freely program, call its set working parameters, easy to use, improve work efficiency

◆15 kinds of acceleration /15 kinds of deceleration options, users can arbitrarily set one group of speed up and down according to sample requirements

◆ With constant speed timing (atsetrpm), start timing two functions, the point is instantaneous centrifugal (short spin) function

◆ Differential centrifugation: Reserve 5 differential centrifugation procedures, users can set their own double-layer steel structure, stainless steel centrifugal chamber, to avoid corrosive substances

◆ Automatic balance, unbalanced protection; Error or imbalance alarm signal prompts, and automatic stop

◆ parameters can be arbitrarily changed during operation, without stopping

Fastest speed up and down time ≤25s

◆ SOFT has soft brake function, which can effectively prevent sample resuspension and protect sensitive samples: multi-stage damping damping design to ensure sample centrifugation effect

◆ Countdown function: countdown in seconds, real-time display of remaining working time

◆ Customizable 485 communication interface, remote control [optional]

◆ Sunken exhaust channel to prevent aerosol pollution during centrifugation

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03Online Message

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1. Sample Pretreatment and Loading Logic

2. Directional Driving Effect of Centrifugal Force

3. Core Mechanism of Uniform Cell Spreading

4. Connection Between Termination and Sample Fixation

Adaptability Between Key Designs and Principles