Calibration specification for ELISA Reader

TheELISA Reader,as the core equipment of immune detection,directly determines the reliability of the results based on its measurement accuracy. Calibration must strictly follow JJF 2089-2023"Calibration Specification for Fully Automated Enzyme Linked Immunoassay Analyzer",and be implemented from three aspects:preparation,operation,and cycle management,as follows:

　　1、Preparation before calibration:strict control of conditions to ensure the foundation

Environment and instrument debugging:It should be carried out in an environment with a temperature of 15-30℃and a relative humidity of≤85%,avoiding direct sunlight and severe vibration. Preheat the instrument for at least 30 minutes before starting up,wipe the sample room's optical path glass with a dust-free cloth,and remove any dust obstruction.

Preparation of tools and reference materials:equipped with an electronic balance with a division value of 0.01mg and a thermometer with a maximum allowable error of±0.1℃; Select a spectral neutral filter(with standard absorbance values covering 0.2-2.0)or a certified standard solution(such as potassium dichromate solution),and use a clean and scratch free blank microplate as an auxiliary tool.

　　2、Core calibration process:step-by-step operation,precise correction

Baseline calibration:Place the blank microplate into the sample chamber for alignment and positioning. Select the commonly used detection wavelength(such as 450nm,630nm)on the operation interface to perform blank calibration. The instrument automatically sets the blank absorbance to"0"to eliminate substrate interference.

Key parameter calibration:

Absorbance calibration:Place the standard filter in a dedicated card slot or microplate hole,input the corresponding wavelength standard value,and the instrument will automatically calculate and store the calibration coefficient after reading the actual value,covering all commonly used wavelengths;

Performance verification:After calibration,retest the calibration material to ensure that the absorbance indication error is≤±0.03,the repeatability is≤1.0%,the channel difference is≤0.03,and the linear correlation coefficient r is≥0.996.

　　3、Calibration cycle management:regular verification,dynamic adjustment

Regular cycle:Regular use(1-3 times a week)calibrated once every 6 months,high-frequency use(daily or weekly testing of over 500 holes)shortened to 3 months,and critical wavelengths need to be checked with standard filters every week.

Special calibration timing:After the instrument is moved or repaired,or if there is a linear deviation in the standard curve or quality control loss of control,it needs to be calibrated immediately; When switching to high-precision detection or uncommon wavelengths,recalibration is also required.

　　4、Attention:Detail control to ensure effectiveness

Wear dust-free gloves when taking the filter to avoid fingerprint contamination affecting the reading; After calibration,record the date,personnel,standard substance information and results in detail,and form a file for archiving. If multiple calibrations still exceed the standard,it is necessary to contact professional personnel to repair the optical path or replace the light source components.